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| Hwang,Jung Eun; Hwang,Sun-Goo; Jung,In Jung; Han,Sung Min; Ahn,Joon-Woo; Kim,Jin-Baek. |
| Abstract Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, ion beams, and cosmic rays. In order to characterize the radiation-responsive genes in rice, OsFBX322 was selected for further analysis. OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR. Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus. To determine the effect of OsFBX322... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: F-box protein; Gamma-irradiation; Ionizing radiation; Rice; Transgenic plant. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100304 |
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| Jung,Yu-Jin; Choi,Chang-Sun; Park,Jin-Heui; Kang,Hee-Wan; Choi,Je-Eul; Nou,Ill-Sup; Lee,Soon Youl; Kang,Kwon-Kyoo. |
| Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1 (the gene encoding fruit bromelain) into a plant expression vector that was then used to transform Brassica rapa and overexpress BAA1 under the control of the cauliflower mosaic virus (CaMV) 35S promoter. We demonstrate that constitutive overexpression of BAA1 in B. rapa confers enhanced resistance to the soft rot pathogen Pectobacterium carotovorum ssp. carotovorum. These results suggest that it could be utilized for protecting plants from attack by bacterial pathogens |
| Tipo: Journal article |
Palavras-chave: Brassica rapa; Fruit bromelain; Soft rot resistance; Transgenic plant. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100007 |
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